bacterialtransformationefficiencycalculator

Transformation Efficiency Calculator ; Total vol. of transformation rxn. ; (μl of recovery medium + μl of cells) ; Plated volume

To calculate the transformation efficiency, we need a few pieces of data: ... Count the number of colonies on the transformation plate. A convenient method to ...

2017年7月17日 — Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 µg of plasmid ...

Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 µg of plasmid into a given volume of ...

This calculation assumes that no futher dilutions of the transformation reaction are made after DNA, competent cells, and media are added.